Hotspot

trnfM-CAU-trnG-UCC

Genome · 42354-42501 · 148 bp

Back to Pohlia

Pi 0.0608
MarkerSeek score 81.40
Primer available yes
Label trnfM-CAU-trnG-UCC

Feature Metadata

label_name
trnfM-CAU-trnG-UCC
feature_type
igs
region
Genome
length_bp
148
variable_sites
13
pi
0.0608
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-CAU-trnG-UCC_p1 TAGCTCGCAAGGCTCATAAC AATATGCGGGTTCGATTCCC 50.0 50.0 57.8 58.1 228-230 bp 1.0 84.0
trnfM-CAU-trnG-UCC_p2 GTAGCTCGCAAGGCTCATAA AATATGCGGGTTCGATTCCC 50.0 50.0 57.8 58.1 229-231 bp 1.0 83.9
trnfM-CAU-trnG-UCC_p3 GTAGCTCGCAAGGCTCATAA AAGGAGAATATGCGGGTTCG 50.0 50.0 57.8 57.8 235-237 bp 1.0 82.7
trnfM-CAU-trnG-UCC_p4 TAGCTCGCAAGGCTCATAAC AAGGAGAATATGCGGGTTCG 50.0 50.0 57.8 57.8 234-236 bp 1.0 82.7
trnfM-CAU-trnG-UCC_p5 TAGCTCGCAAGGCTCATAAC TTGCCAAGGAGAATATGCGG 50.0 50.0 57.8 58.3 239-241 bp 1.0 82.0