Hotspot

ndhJ-trnF-GAA

Genome · 32713-32932 · 220 bp

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Pi 0.0645
MarkerSeek score 78.90
Primer available yes
Label ndhJ-trnF-GAA

Feature Metadata

label_name
ndhJ-trnF-GAA
feature_type
igs
region
Genome
length_bp
220
variable_sites
20
pi
0.0645
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhJ-trnF-GAA_p1 TTTTTATGAGTTACAAGATGCTT AAGCCGGAATAGCTCAGTTG 26.1 50.0 52.6 58.0 309-323 bp 1.0 48.6
ndhJ-trnF-GAA_p2 GATTTTTATGAGTTACAAGATGCT AAGCCGGAATAGCTCAGTTG 29.2 50.0 53.7 58.0 311-325 bp 1.0 48.5
ndhJ-trnF-GAA_p3 GATTTTTATGAGTTACAAGATGCTT AAGCCGGAATAGCTCAGTTG 28.0 50.0 54.4 58.0 311-325 bp 1.0 48.5
ndhJ-trnF-GAA_p4 ATTTTTATGAGTTACAAGATGCT AAGCCGGAATAGCTCAGTTG 26.1 50.0 52.4 58.0 310-324 bp 1.0 48.5
ndhJ-trnF-GAA_p5 GATTTTTATGAGTTACAAGATGC AAGCCGGAATAGCTCAGTTG 30.4 50.0 52.3 58.0 311-325 bp 1.0 48.5