Hotspot

psbJ-petA

Genome · 19669-20664 · 996 bp

Pi 0.0505

MarkerSeek score 76.50

Primer available yes

Label psbJ-petA

Feature Metadata

label_name: psbJ-petA
feature_type: igs
region: Genome
length_bp: 996
variable_sites: 75
pi: 0.0505
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-petA_p1	ATGACGTTTTTGGAAGCCCT	ACCTTCAGGACCCGAACTTA	45.0	50.0	58.0	58.0	1878-1880 bp	1.0	84.1
psbJ-petA_p2	ATGACGTTTTTGGAAGCCCT	TACCTTCAGGACCCGAACTT	45.0	50.0	58.0	58.0	1879-1881 bp	1.0	84.1
psbJ-petA_p3	ATGACGTTTTTGGAAGCCCT	CATTGTACCTTCAGGACCCG	45.0	55.0	58.0	58.0	1884-1886 bp	1.0	84.1
psbJ-petA_p4	ATGACGTTTTTGGAAGCCCT	ATTGTACCTTCAGGACCCGA	45.0	50.0	58.0	58.1	1883-1885 bp	1.0	83.9
psbJ-petA_p5	GTGCATGGACTAGCTGTACC	ACCTTCAGGACCCGAACTTA	55.0	50.0	58.1	58.0	1699-1701 bp	0.666667	69.1