Hotspot

rpl16

LSC · 80646-81996 · 1351 bp

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Pi 0.0212

MarkerSeek score 61.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1351
variable_sites: 51
pi: 0.0212
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACCGCTGTCACGTTTAAGTT	ATTGCACGTGTCGAATGGAT	45.0	45.0	58.1	58.3	1967-1997 bp	1.0	81.9
rpl16_p2	ACCGCTGTCACGTTTAAGTT	CACGTGTCGAATGGATCAGA	45.0	50.0	58.1	57.7	1963-1993 bp	1.0	81.8
rpl16_p3	ACCGCTGTCACGTTTAAGTT	CAAGTACAAATTGCAGGGCG	45.0	50.0	58.1	58.0	2005-2033 bp	0.6	65.3
rpl16_p4	CCGCTGTTATCCGCTACATT	CAAGTACAAATTGCAGGGCG	50.0	50.0	58.1	58.0	1827-1855 bp	0.6	64.8
rpl16_p5	TGTCTCAATTCTCGGGGGAT	CAAGTACAAATTGCAGGGCG	50.0	50.0	58.1	58.0	2106-2134 bp	0.6	64.7