Hotspot

psaC-ndhE

SSC · 116013-116264 · 252 bp

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Pi 0.0444

MarkerSeek score 73.40

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 252
variable_sites: 21
pi: 0.0444
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	TACATCCGTCCCAGGGTATC	TCGTAACAGAAAATCAACTCGT	55.0	36.4	58.0	56.1	407 bp	1.0	52.7
psaC-ndhE_p2	CTAATACATCCGTGGGGCAC	TCGTAACAGAAAATCAACTCGT	55.0	36.4	58.1	56.1	383 bp	1.0	52.1
psaC-ndhE_p3	TCCGTCCCAGGGTATCATTT	TCGTAACAGAAAATCAACTCGT	50.0	36.4	58.1	56.1	403 bp	1.0	51.9
psaC-ndhE_p4	ATCCGTCCCAGGGTATCATT	TCGTAACAGAAAATCAACTCGT	50.0	36.4	57.9	56.1	404 bp	1.0	51.9
psaC-ndhE_p5	TCTAATACATCCGTGGGGCA	TCGTAACAGAAAATCAACTCGT	50.0	36.4	58.2	56.1	384 bp	1.0	51.4