Hotspot

ndhF-rpl32

SSC · 111413-111909 · 497 bp

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Pi 0.041

MarkerSeek score 72.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 497
variable_sites: 38
pi: 0.041
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CAGAAGGTCGTGTGAACCAA	AAATGCTTTTAACGCTGCCC	50.0	45.0	58.1	57.9	1099-1110 bp	1.0	83.5
ndhF-rpl32_p2	CAGAAGGTCGTGTGAACCAA	AATAAGAACCCCACCCTGGA	50.0	50.0	58.1	57.9	1456-1460 bp	0.6	64.4
ndhF-rpl32_p3	CAGAAGGTCGTGTGAACCAA	ATAAGAACCCCACCCTGGAA	50.0	50.0	58.1	57.9	1455-1459 bp	0.6	64.4
ndhF-rpl32_p4	CAGAAGGTCGTGTGAACCAA	CCATTTCCTTCAACGGGGAA	50.0	50.0	58.1	58.1	1298 bp	0.2	46.9
ndhF-rpl32_p5	CAGAAGGTCGTGTGAACCAA	TTTCCTTCAACGGGGAATGG	50.0	50.0	58.1	58.1	1295 bp	0.2	46.9