Hotspot

atpA-atpF

LSC · 11940-12040 · 101 bp

Back to Pogostemon

Pi 0.0475

MarkerSeek score 76.10

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 101
variable_sites: 10
pi: 0.0475
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CACCCGCCATTACTTCATCA	GTAATTGAGCGTGAGAGCCA	50.0	50.0	58.0	58.0	753-759 bp	1.0	82.4
atpA-atpF_p2	ATTCGACTAATTCACCCGCC	GTAATTGAGCGTGAGAGCCA	50.0	50.0	58.1	58.0	765-771 bp	1.0	82.2
atpA-atpF_p3	ATACGAGCAATACCATCGCC	GTAATTGAGCGTGAGAGCCA	50.0	50.0	57.9	58.0	724-730 bp	1.0	82.0
atpA-atpF_p4	CCGTTTCGGCTACCACAATA	GTAATTGAGCGTGAGAGCCA	50.0	50.0	58.0	58.0	1284-1290 bp	1.0	81.8
atpA-atpF_p5	CTCATATACTGAACGCCGGG	GTAATTGAGCGTGAGAGCCA	55.0	50.0	57.9	58.0	1034-1040 bp	1.0	81.6