Hotspot

rps16-trnQ-UUG

LSC · 6217-7363 · 1147 bp

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Pi 0.0325

MarkerSeek score 77.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1147
variable_sites: 72
pi: 0.0325
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GTCTTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1181-1264 bp	1.0	87.8
rps16-trnQ-UUG_p2	CAGCGGATCCTGTCTTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1192-1275 bp	1.0	86.9
rps16-trnQ-UUG_p3	GCGGATCCTGTCTTTCAAGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1190-1273 bp	1.0	86.9
rps16-trnQ-UUG_p4	TCCTGTCTTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.1	58.1	1185-1268 bp	1.0	86.7
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1174-1257 bp	1.0	86.7