Hotspot

trnK-UUU-rps16

LSC · 4181-5069 · 889 bp

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Pi 0.0289
MarkerSeek score 70.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
889
variable_sites
52
pi
0.0289
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AGGAACTTCCGCCTAATCAAC 50.0 47.6 58.2 58.0 953-970 bp 1.0 76.9
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT GAACTTCCGCCTAATCAACCT 50.0 47.6 58.2 58.0 951-968 bp 1.0 76.9
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT AGGAACTTCCGCCTAATCAAC 50.0 47.6 58.2 58.0 952-969 bp 1.0 76.9
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT GAACTTCCGCCTAATCAACCT 50.0 47.6 58.2 58.0 950-967 bp 1.0 76.9
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT AACTTCCGCCTAATCAACCT 50.0 45.0 58.2 56.8 949-966 bp 1.0 75.6