Hotspot

rpoA

LSC · 81303-82322 · 1020 bp

Back to Pogonia

Pi 0.00098

MarkerSeek score 59.30

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1020
variable_sites: 1
pi: 0.00098
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	ACCGAAGATTCCAAACTCAT	AGACGTGTGTAAAATTCAAGAGA	40.0	34.8	54.7	56.2	1222-1223 bp	1.0	40.1
rpoA_p2	TCATACCGAAGATTCCAAACTCA	AGACGTGTGTAAAATTCAAGAGA	39.1	34.8	57.6	56.2	1226-1227 bp	1.0	40.1
rpoA_p3	ACCGAAGATTCCAAACTCATT	AGACGTGTGTAAAATTCAAGAGA	38.1	34.8	55.5	56.2	1222-1223 bp	1.0	40.1
rpoA_p4	ACCGAAGATTCCAAACTCAT	ACGTGTGTAAAATTCAAGAGATTCC	40.0	36.0	54.7	58.1	1220-1221 bp	1.0	40.1
rpoA_p5	ACCGAAGATTCCAAACTCAT	CGTGTGTAAAATTCAAGAGATTCCA	40.0	36.0	54.7	58.1	1219-1220 bp	1.0	40.1