Hotspot

petD

LSC · 79641-81100 · 1460 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1460
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TGGATTATTTCAAAAATAAGGCATGT	AGGGAAGATCCAGAAAAGGA	26.9	45.0	56.4	55.4	1631 bp	1.0	40.0
petD_p2	AGTCTATTTTTCTAAAAGAGGATGGA	AGGGAAGATCCAGAAAAGGA	30.8	45.0	56.1	55.4	1544 bp	1.0	40.0
petD_p3	TGGATTATTTCAAAAATAAGGCATGTT	AGGGAAGATCCAGAAAAGGA	25.9	45.0	57.0	55.4	1631 bp	1.0	40.0
petD_p4	TGGATTATTTCAAAAATAAGGCATGT	TCTAGGGAAGATCCAGAAAAGGA	26.9	43.5	56.4	58.0	1634 bp	1.0	40.0
petD_p5	TGGATTATTTCAAAAATAAGGCATG	AGGGAAGATCCAGAAAAGGA	28.0	45.0	54.9	55.4	1631 bp	1.0	40.0