Hotspot

ndhA

IRb · 119689-120284 · 596 bp

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Pi 0.00524

MarkerSeek score 65.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: IRb
length_bp: 596
variable_sites: 3
pi: 0.00524
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ATGGAATCTGTGGCGTTAGC	TGAAGTCAAATAAATTGCCATCCA	50.0	33.3	58.3	57.4	678-702 bp	1.0	43.7
ndhA_p2	GCGTTAGCCCATGGGATTTT	TGAAGTCAAATAAATTGCCATCCA	50.0	33.3	58.9	57.4	666-690 bp	1.0	43.2
ndhA_p3	ATGGAATCTGTGGCGTTAGC	AGTCAAATAAATTGCCATCCAAGA	50.0	33.3	58.3	57.1	675-699 bp	1.0	43.1
ndhA_p4	ATGGAATCTGTGGCGTTAGC	TGAAGTCAAATAAATTGCCATCCAA	50.0	32.0	58.3	58.0	678-702 bp	1.0	43.1
ndhA_p5	ATGGAATCTGTGGCGTTAGC	TTGAAGTCAAATAAATTGCCATCCA	50.0	32.0	58.3	58.0	679-703 bp	1.0	43.1