Hotspot

accD

LSC · 59123-60763 · 1641 bp

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Pi 0
MarkerSeek score 59.70
Primer available yes
Label accD

Feature Metadata

label_name
accD
feature_type
gene
region
LSC
length_bp
1641
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD_p1 TTAGGGGACAGAAAGGCTCT ACGTGCTACAACTACAAAGAAGT 50.0 39.1 57.7 58.3 1718-1739 bp 1.0 51.7
accD_p2 TTAGGGGACAGAAAGGCTCT ACGTGCTACAACTACAAAGAAG 50.0 40.9 57.7 56.7 1718-1739 bp 1.0 51.7
accD_p3 TTAGGGGACAGAAAGGCTCT CGTGCTACAACTACAAAGAAGT 50.0 40.9 57.7 56.7 1717-1738 bp 1.0 51.7
accD_p4 TTAGGGGACAGAAAGGCTCT ACGTGCTACAACTACAAAGA 50.0 40.0 57.7 54.7 1718-1739 bp 1.0 51.7
accD_p5 TTAGGGGACAGAAAGGCTCT ACGTGCTACAACTACAAAGAA 50.0 38.1 57.7 55.5 1718-1739 bp 1.0 50.3