Hotspot

ndhD-ccsA

SSC · 122126-122438 · 313 bp

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Pi 0

MarkerSeek score 40.40

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 313
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 40.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	TTCTCGATGGCTTCTCTTGC	GTCCAAGGCACAAATTCAGC	50.0	50.0	58.0	57.9	622-629 bp	1.0	80.3
ndhD-ccsA_p2	CTCGATGGCTTCTCTTGCAT	GTCCAAGGCACAAATTCAGC	50.0	50.0	58.0	57.9	620-627 bp	1.0	80.1
ndhD-ccsA_p3	TTCTCGATGGCTTCTCTTGC	TTTTACCCAAGGTTTCGCCA	50.0	45.0	58.0	57.9	1100-1107 bp	1.0	79.9
ndhD-ccsA_p4	TTCTCGATGGCTTCTCTTGC	AGGCATTCTTTCTGGAGCAG	50.0	50.0	58.0	57.9	761-768 bp	1.0	79.7
ndhD-ccsA_p5	CTCGATGGCTTCTCTTGCAT	TTTTACCCAAGGTTTCGCCA	50.0	45.0	58.0	57.9	1098-1105 bp	1.0	79.6