Hotspot

rbcL-accD

LSC · 58248-59122 · 875 bp

Back to Pogonia

Pi 0.00114

MarkerSeek score 59.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 875
variable_sites: 1
pi: 0.00114
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CTAGCAAATGGAGCCCTGAG	CCCGCTAGCATCAAGACTAC	55.0	55.0	58.0	57.9	1063 bp	1.0	78.7
rbcL-accD_p2	CTAGCAAATGGAGCCCTGAG	CCGCTAGCATCAAGACTACC	55.0	55.0	58.0	57.9	1062 bp	1.0	78.7
rbcL-accD_p3	CTAGCAAATGGAGCCCTGAG	CGCTAGCATCAAGACTACCC	55.0	55.0	58.0	57.9	1061 bp	1.0	78.7
rbcL-accD_p4	TGAAGCTAGCAAATGGAGCC	CCGCTAGCATCAAGACTACC	50.0	55.0	58.2	57.9	1067 bp	1.0	77.0
rbcL-accD_p5	TGAAGCTAGCAAATGGAGCC	CGCTAGCATCAAGACTACCC	50.0	55.0	58.2	57.9	1066 bp	1.0	77.0