Hotspot

psbD-trnT(GGU)

LSC · 21367-22301 · 935 bp

Back to Pogonia

Pi 0.00107

MarkerSeek score 59.40

Primer available yes

Label psbD-trnT(GGU)

Feature Metadata

label_name: psbD-trnT(GGU)
feature_type: igs
region: LSC
length_bp: 935
variable_sites: 1
pi: 0.00107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT(GGU)_p1	AGGAAAGAGCAATATGCCGG	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.0	58.1	1113 bp	1.0	79.3
psbD-trnT(GGU)_p2	GGAAAGAGCAATATGCCGGA	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.0	58.1	1112 bp	1.0	79.3
psbD-trnT(GGU)_p3	AGGAAAGAGCAATATGCCGG	TCAGTGGTAGAGTAACGCCA	50.0	50.0	58.0	58.1	1116 bp	1.0	79.2
psbD-trnT(GGU)_p4	GGAAAGAGCAATATGCCGGA	TCAGTGGTAGAGTAACGCCA	50.0	50.0	58.0	58.1	1115 bp	1.0	79.1
psbD-trnT(GGU)_p5	GGACCAGCCTACAAAAACGA	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.1	58.1	1095 bp	1.0	78.7