Hotspot

matK-trnK(UUU)

LSC · 3468-4791 · 1324 bp

Back to Pogonia

Pi 0.000758
MarkerSeek score 59.60
Primer available yes
Label matK-trnK(UUU)

Feature Metadata

label_name
matK-trnK(UUU)
feature_type
igs
region
LSC
length_bp
1324
variable_sites
1
pi
0.000758
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK(UUU)_p1 TCTTGCAGGAGAAGCGGATA GGTCCAGTTGATCAGTGCAA 50.0 50.0 58.5 58.1 1512-1558 bp 1.0 75.4
matK-trnK(UUU)_p2 TCTTGCAGGAGAAGCGGATA TTGGTCCAGTTGATCAGTGC 50.0 50.0 58.5 58.1 1514-1560 bp 1.0 75.4
matK-trnK(UUU)_p3 CTTGCAGGAGAAGCGGATAT GGTCCAGTTGATCAGTGCAA 50.0 50.0 57.5 58.1 1511-1557 bp 1.0 75.2
matK-trnK(UUU)_p4 CTTGCAGGAGAAGCGGATAT TTGGTCCAGTTGATCAGTGC 50.0 50.0 57.5 58.1 1513-1559 bp 1.0 75.2
matK-trnK(UUU)_p5 TCTTGCAGGAGAAGCGGATA ACGGTAGAGTACTCGGCTTT 50.0 50.0 58.5 58.2 1433-1478 bp 1.0 74.9