Hotspot

rpoC1

Genome · 11752-14253 · 2502 bp

Back to Podocarpus

Pi 0.0184
MarkerSeek score 61.00
Primer available yes
Label rpoC1

Feature Metadata

label_name
rpoC1
feature_type
gene
region
Genome
length_bp
2502
variable_sites
111
pi
0.0184
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC1_p1 GGAAGGCTTCCTCGATCTCT AACCAGACACTACTCCCGAA 55.0 50.0 58.6 58.0 2667-2810 bp 1.0 82.9
rpoC1_p2 GGAAGGCTTCCTCGATCTCT AGTTTGGGCTCTAGAAGGGT 55.0 50.0 58.6 58.0 2798-2941 bp 1.0 82.4
rpoC1_p3 GGAAGGCTTCCTCGATCTCT AGGAAGATCCAGACGAGGAG 55.0 55.0 58.6 57.9 2840-2983 bp 1.0 82.2
rpoC1_p4 GGAAGGCTTCCTCGATCTCT GCGCCATTCTTACTGGAGAA 55.0 50.0 58.6 58.0 2703-2846 bp 0.833333 74.5
rpoC1_p5 GGAAGGCTTCCTCGATCTCT GGTTACGCAACAGCCTCTTA 55.0 50.0 58.6 57.9 2861-2993 bp 0.666667 67.8