Hotspot

psbA-trnK-UUU

Genome · 125397-125888 · 492 bp

Back to Podocarpus

Pi 0.0247

MarkerSeek score 73.00

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: Genome
length_bp: 492
variable_sites: 32
pi: 0.0247
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	GCTAGTGATCCAGTCGCAAA	TAATCCACTCCATCCGACGA	50.0	50.0	58.0	57.9	602-613 bp	1.0	84.8
psbA-trnK-UUU_p2	GTGATCCAGTCGCAAAAACG	TAATCCACTCCATCCGACGA	50.0	50.0	58.0	57.9	598-609 bp	1.0	84.5
psbA-trnK-UUU_p3	TGATCCAGTCGCAAAAACGA	TAATCCACTCCATCCGACGA	45.0	50.0	58.1	57.9	597-608 bp	1.0	83.9
psbA-trnK-UUU_p4	GCTAGTGATCCAGTCGCAAA	GAAAGCCGTGTGCAATGAAA	50.0	45.0	58.0	57.9	673-684 bp	1.0	83.7
psbA-trnK-UUU_p5	GTGATCCAGTCGCAAAAACG	GAAAGCCGTGTGCAATGAAA	50.0	45.0	58.0	57.9	669-680 bp	1.0	83.4