Hotspot

ycf2-trnI-CAU

Genome · 119789-120217 · 429 bp

Back to Podocarpus

Pi 0.0527

MarkerSeek score 82.50

Primer available yes

Label ycf2-trnI-CAU

Feature Metadata

label_name: ycf2-trnI-CAU
feature_type: igs
region: Genome
length_bp: 429
variable_sites: 55
pi: 0.0527
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnI-CAU_p1	GCGTTTGTCTCGGGTCAT	CCGCGACTTCCCAATTATGA	55.6	50.0	57.7	58.1	469-602 bp	1.0	73.4
ycf2-trnI-CAU_p2	GCGTTTGTCTCGGGTCAT	GGGTGCTTTAACCATTCAGC	55.6	50.0	57.7	57.3	446-579 bp	1.0	68.5
ycf2-trnI-CAU_p3	GCGTTTGTCTCGGGTCAT	GGTGCTTTAACCATTCAGCC	55.6	50.0	57.7	57.3	445-578 bp	1.0	68.5
ycf2-trnI-CAU_p4	GCGTTTGTCTCGGGTCAT	TGAGTTGGGTGCTTTAACCA	55.6	45.0	57.7	57.3	452-585 bp	1.0	67.9
ycf2-trnI-CAU_p5	GCGTTTGTCTCGGGTCAT	ATTGGAAGACCTGCATCCAG	55.6	50.0	57.7	57.6	512-634 bp	0.833333	61.4