Hotspot

rrn16-trnV-GAC

Genome · 105743-107263 · 1521 bp

Back to Podocarpus

Pi 0.055

MarkerSeek score 89.60

Primer available yes

Label rrn16-trnV-GAC

Feature Metadata

label_name: rrn16-trnV-GAC
feature_type: igs
region: Genome
length_bp: 1521
variable_sites: 195
pi: 0.055
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 89.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-trnV-GAC_p1	CATTGCGGAAAATTCCCCAC	TCCGAAAATTCTTCACCGCA	50.0	45.0	58.0	58.1	2312-2377 bp	1.0	90.8
rrn16-trnV-GAC_p2	ATTCCGGATAACGCTTGCAT	TCCGAAAATTCTTCACCGCA	45.0	45.0	58.0	58.1	2474-2539 bp	1.0	90.1
rrn16-trnV-GAC_p3	TGCCCCTACCATACTCTAGC	TCCGAAAATTCTTCACCGCA	55.0	45.0	58.0	58.1	2582-2647 bp	1.0	90.0
rrn16-trnV-GAC_p4	TCGTCTCTCAGTGTCAGTGT	TCCGAAAATTCTTCACCGCA	50.0	45.0	58.0	58.1	2679-2744 bp	1.0	89.7
rrn16-trnV-GAC_p5	CGTAGCCTTGGTAAGCCATT	TCCGAAAATTCTTCACCGCA	50.0	45.0	58.0	58.1	2217-2240 bp	0.833333	83.2