Hotspot

psbB-clpP

Genome · 73502-74280 · 779 bp

Back to Podocarpus

Pi 0.0396

MarkerSeek score 80.60

Primer available yes

Label psbB-clpP

Feature Metadata

label_name: psbB-clpP
feature_type: igs
region: Genome
length_bp: 779
variable_sites: 80
pi: 0.0396
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-clpP_p1	CCCAACCGGAAACTAAAGCT	GTCCAAGTACTAGCGGGTTG	50.0	55.0	58.1	58.0	1258-1277 bp	1.0	88.5
psbB-clpP_p2	AGCCCAACCGGAAACTAAAG	GTCCAAGTACTAGCGGGTTG	50.0	55.0	58.1	58.0	1260-1279 bp	1.0	88.4
psbB-clpP_p3	AGGACGAACACTGAGATGGA	GTCCAAGTACTAGCGGGTTG	50.0	55.0	58.1	58.0	1827-1840 bp	0.833333	78.1
psbB-clpP_p4	AGGACGAACACTGAGATGGA	AGGACCCTGTTTCCGTTTTC	50.0	50.0	58.1	58.0	2068-2081 bp	0.666667	69.9
psbB-clpP_p5	AGGACGAACACTGAGATGGA	GGACCCTGTTTCCGTTTTCT	50.0	50.0	58.1	58.0	2067-2080 bp	0.666667	69.9