Hotspot

trnF-GAA-ndhJ

Genome · 37866-38379 · 514 bp

Back to Podocarpus

Pi 0.0246

MarkerSeek score 69.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: Genome
length_bp: 514
variable_sites: 29
pi: 0.0246
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	AGGACTGAAAATCCTCGTGC	TTGGCCTTTACGCAAAGACT	50.0	45.0	57.9	58.0	616-632 bp	1.0	84.4
trnF-GAA-ndhJ_p2	AGGACTGAAAATCCTCGTGC	GTTGGCCTTTACGCAAAGAC	50.0	50.0	57.9	58.0	617-633 bp	1.0	84.2
trnF-GAA-ndhJ_p3	AGGACTGAAAATCCTCGTGC	TGAGAGTTGGATTGGTTGGC	50.0	50.0	57.9	58.1	631-647 bp	1.0	83.8
trnF-GAA-ndhJ_p4	AGGACTGAAAATCCTCGTGC	AGTTGGATTGGTTGGCCTTT	50.0	45.0	57.9	57.9	627-643 bp	1.0	83.7
trnF-GAA-ndhJ_p5	GACTGAAAATCCTCGTGCCA	TTGGCCTTTACGCAAAGACT	50.0	45.0	58.2	58.0	614-630 bp	1.0	83.7