Hotspot

ndhF

SSC · 101822-104056 · 2235 bp

Pi 0.00672

MarkerSeek score 48.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2235
variable_sites: 37
pi: 0.00672
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AAAATCGCGGAAAACCGAAC	GGTACGGAAGCCTATCTCCT	45.0	55.0	58.0	58.0	2943-2981 bp	0.5	60.8
ndhF_p2	AATCGCGGAAAACCGAACTA	GGTACGGAAGCCTATCTCCT	45.0	55.0	57.9	58.0	2941-2979 bp	0.5	60.5
ndhF_p3	TCGCGGAAAACCGAACTATT	GGTACGGAAGCCTATCTCCT	45.0	55.0	57.9	58.0	2939-2977 bp	0.5	60.5
ndhF_p4	AAGGGGGAGGGGATAGGATA	GGTACGGAAGCCTATCTCCT	55.0	55.0	58.1	58.0	2872-2885 bp	0.5	59.6
ndhF_p5	ATAGGAAGGGGGAGGGGATA	GGTACGGAAGCCTATCTCCT	55.0	55.0	58.1	58.0	2877-2890 bp	0.5	59.6