Hotspot

trnD-GUC-psbM

LSC · 16073-16767 · 695 bp

Pi 0.0125

MarkerSeek score 61.10

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 695
variable_sites: 20
pi: 0.0125
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TTCAATTGGTCAGAGCACCG	AGAATGAACAGTGCAGTAGC	50.0	45.0	58.5	55.8	797-819 bp	1.0	61.3
trnD-GUC-psbM_p2	TAGGTATGCCATACACCCCG	AGAATGAACAGTGCAGTAGC	55.0	45.0	58.7	55.8	829-851 bp	1.0	59.8
trnD-GUC-psbM_p3	GTTCAATTGGTCAGAGCACC	AGAATGAACAGTGCAGTAGC	50.0	45.0	57.0	55.8	798-820 bp	1.0	57.2
trnD-GUC-psbM_p4	CGCGGGGATTGTAGTTCAA	AGAATGAACAGTGCAGTAGC	52.6	45.0	57.9	55.8	811-833 bp	1.0	55.9
trnD-GUC-psbM_p5	TCAATTGGTCAGAGCACCG	AGAATGAACAGTGCAGTAGC	52.6	45.0	57.8	55.8	796-818 bp	1.0	55.3