Hotspot

trnG-UCC-trnT-GGU

LSC · 13580-14813 · 1234 bp

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Pi 0.0122
MarkerSeek score 45.20
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1234
variable_sites
34
pi
0.0122
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.75
markerseek_score
45.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 ACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 38.1 45.0 55.5 56.6 1066-1332 bp 1.0 47.0
trnG-UCC-trnT-GGU_p2 ACGAATCACACTTTTACCACT CCGATGACTTATGCCTTACCA 38.1 47.6 55.5 57.5 1062-1328 bp 1.0 46.3
trnG-UCC-trnT-GGU_p3 ACGAATCACACTTTTACCACT ACCGATGACTTATGCCTTACC 38.1 47.6 55.5 57.5 1063-1329 bp 1.0 46.3
trnG-UCC-trnT-GGU_p4 AGAACGAATCACACTTTTACCAC TGAACCGATGACTTATGCCT 39.1 45.0 57.3 56.6 1069-1335 bp 1.0 46.3
trnG-UCC-trnT-GGU_p5 GAACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 39.1 45.0 57.3 56.6 1068-1334 bp 1.0 46.3