Hotspot

ndhD

SSC · 116427-117929 · 1503 bp

Back to Plumeria

Pi 0.00177

MarkerSeek score 46.20

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 4
pi: 0.00177
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	ACGAACCGTGCGAATCAATA	CTGCCCGACCGATGTATTAG	45.0	55.0	58.0	57.9	2004 bp	1.0	80.2
ndhD_p2	ACGAACCGTGCGAATCAATA	TGTGAATCTGCCTGTCCAAC	45.0	50.0	58.0	58.1	1904 bp	1.0	79.9
ndhD_p3	ACGAACCGTGCGAATCAATA	TGATACCTTGGGACGGATGT	45.0	50.0	58.0	58.1	1981 bp	1.0	79.7
ndhD_p4	ACGAACCGTGCGAATCAATA	GGAAGACTGCGTTGGTTGTA	45.0	50.0	58.0	58.1	1929 bp	1.0	79.7
ndhD_p5	ACGAACCGTGCGAATCAATA	ACAAAACCCGTGCTCAAGAT	45.0	45.0	58.0	58.0	1771 bp	0.666667	66.6