Hotspot

rpl23-rpl2

IRa · 152672-152689 · 18 bp

Back to Plumeria

Pi 0.037

MarkerSeek score 63.60

Primer available yes

Label rpl23-rpl2

Feature Metadata

label_name: rpl23-rpl2
feature_type: igs
region: IRa
length_bp: 18
variable_sites: 1
pi: 0.037
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-rpl2_p1	CGCTTCAACCGGGTTATTCT	CCACAACGATGCTGTCCATA	50.0	50.0	58.3	58.0	181 bp	1.0	78.1
rpl23-rpl2_p2	CGCTTCAACCGGGTTATTCT	ACTGTCTACGGTTCCATTGC	50.0	50.0	58.3	57.9	128 bp	1.0	77.7
rpl23-rpl2_p3	ATCATTACGCTTCAACCGGG	CCACAACGATGCTGTCCATA	50.0	50.0	58.3	58.0	188 bp	1.0	77.6
rpl23-rpl2_p4	TCCGGGAAAGGGTAGAAGAA	CCACAACGATGCTGTCCATA	50.0	50.0	57.7	58.0	249 bp	1.0	77.5
rpl23-rpl2_p5	TTCCGGGAAAGGGTAGAAGA	CCACAACGATGCTGTCCATA	50.0	50.0	57.7	58.0	250 bp	1.0	77.5