Hotspot

rpl32-trnL-UAG

SSC · 114063-115033 · 971 bp

Back to Plumeria

Pi 0.00893

MarkerSeek score 52.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 971
variable_sites: 13
pi: 0.00893
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGTGCAGCAAACAAATAAGT	TTAGAAGATGACATGCCGCC	35.0	50.0	54.3	58.0	1083-1090 bp	1.0	51.3
rpl32-trnL-UAG_p2	TGTGCAGCAAACAAATAAGT	TCCTAAGAGCAGCGTGTCTA	35.0	50.0	54.3	57.9	1029-1036 bp	1.0	50.7
rpl32-trnL-UAG_p3	TGTGCAGCAAACAAATAAGT	AGCAGCGTGTCTACCAATTT	35.0	45.0	54.3	57.8	1022-1029 bp	1.0	50.2
rpl32-trnL-UAG_p4	TGTGCAGCAAACAAATAAGT	CCTAAGAGCAGCGTGTCTAC	35.0	55.0	54.3	57.8	1028-1035 bp	1.0	50.1
rpl32-trnL-UAG_p5	TGTGCAGCAAACAAATAAGT	CTAAGAGCAGCGTGTCTACC	35.0	55.0	54.3	57.8	1027-1034 bp	1.0	50.1