Hotspot

trnM-CAU-atpE

LSC · 53460-53704 · 245 bp

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Pi 0.0181
MarkerSeek score 67.00
Primer available yes
Label trnM-CAU-atpE

Feature Metadata

label_name
trnM-CAU-atpE
feature_type
igs
region
LSC
length_bp
245
variable_sites
6
pi
0.0181
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-atpE_p1 GGCGAGAGTCATTGGTTCAA CGGGTAGAGGCTATCAATGC 50.0 55.0 58.2 57.9 299-307 bp 1.0 81.5
trnM-CAU-atpE_p2 GGCGAGAGTCATTGGTTCAA GGGTAGAGGCTATCAATGCG 50.0 55.0 58.2 57.9 298-306 bp 1.0 81.5
trnM-CAU-atpE_p3 GGCGAGAGTCATTGGTTCAA GGTAGAGGCTATCAATGCGG 50.0 55.0 58.2 57.9 297-305 bp 1.0 81.5
trnM-CAU-atpE_p4 GGCGAGAGTCATTGGTTCAA ACACGGGTAGAGGCTATCAA 50.0 50.0 58.2 57.8 302-310 bp 1.0 80.9
trnM-CAU-atpE_p5 ATTGCTTTCATACGGCGAGA GGTAGAGGCTATCAATGCGG 45.0 55.0 57.7 57.9 310-318 bp 1.0 80.4