Hotspot

ndhC-trnV-UAC

LSC · 51358-52554 · 1197 bp

Back to Plumeria

Pi 0.00585

MarkerSeek score 62.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1197
variable_sites: 10
pi: 0.00585
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	885-1336 bp	1.0	82.0
ndhC-trnV-UAC_p2	CTGGTCCTTTGCTAATCGGG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.3	57.8	902-1353 bp	1.0	80.4
ndhC-trnV-UAC_p3	CCTTTGCTAATCGGGGCTAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	897-1348 bp	1.0	80.4
ndhC-trnV-UAC_p4	GGGGCTAAAACTCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	884-1335 bp	1.0	79.2
ndhC-trnV-UAC_p5	TCCTTTGCTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.5	57.8	898-1349 bp	1.0	79.1