Hotspot

trnT-GGU-psbD

LSC · 32372-33629 · 1258 bp

Back to Plumeria

Pi 0.00372
MarkerSeek score 61.40
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1258
variable_sites
7
pi
0.00372
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GCCCTTTTAACTCAGCGGTA GAAACGGTCCCTACGTAACC 50.0 55.0 57.9 58.0 1414-1419 bp 1.0 79.7
trnT-GGU-psbD_p2 GCCCTTTTAACTCAGCGGTA GGACCAGCCTACAAAAACGA 50.0 50.0 57.9 58.1 1432-1437 bp 1.0 78.8
trnT-GGU-psbD_p3 GGCGTAAGTCATCGGTTCAA GAAACGGTCCCTACGTAACC 50.0 55.0 58.3 58.0 1377-1382 bp 1.0 78.3
trnT-GGU-psbD_p4 GCCCTTTTAACTCAGCGGTA ACAAAAACGAAACGGTCCCT 50.0 45.0 57.9 58.2 1422-1427 bp 1.0 77.8
trnT-GGU-psbD_p5 GGCGTAAGTCATCGGTTCAA GGACCAGCCTACAAAAACGA 50.0 50.0 58.3 58.1 1395-1400 bp 1.0 77.4