Hotspot

ycf1

IRb · 117286-123495 · 6210 bp

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Pi 0.0567

MarkerSeek score 74.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 6210
variable_sites: 504
pi: 0.0567
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCATTCGTAGTTGATCCCGT	TTTCCCTCCTCCAATCCCTT	50.0	50.0	58.1	57.9	1075-1081 bp	0.666667	70.1
ycf1_p2	GCATTCGTAGTTGATCCCGT	TTTTCCCTCCTCCAATCCCT	50.0	50.0	58.1	57.9	1076-1082 bp	0.666667	70.1
ycf1_p3	GCATTCGTAGTTGATCCCGT	TGGTTCCGTGGACTCTAGTT	50.0	50.0	58.1	58.0	894 bp	0.333333	52.8
ycf1_p4	ACATCGGGGCAGAGAAGATA	TGGTTCCGTGGACTCTAGTT	50.0	50.0	57.9	58.0	722 bp	0.333333	52.6
ycf1_p5	CCCCAATGGCCATACAACTT	TGGTTCCGTGGACTCTAGTT	50.0	50.0	58.1	58.0	780 bp	0.333333	52.2