Hotspot

ycf2-trnI-CAU

IRa · 166170-166579 · 410 bp

Back to Plumbago

Pi 0.0634

MarkerSeek score 80.00

Primer available yes

Label ycf2-trnI-CAU

Feature Metadata

label_name: ycf2-trnI-CAU
feature_type: igs
region: IRa
length_bp: 410
variable_sites: 37
pi: 0.0634
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnI-CAU_p1	TTGAAGTGGGTCCATGAAGC	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.1	58.0	573-582 bp	1.0	90.4
ycf2-trnI-CAU_p2	GGAGCGAAACAATCAGCCTA	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.0	58.0	896-905 bp	1.0	87.0
ycf2-trnI-CAU_p3	CCAGGAAACAACTCTCCGAG	GTTGGGCGCTTTAACCATTC	55.0	50.0	57.9	58.0	941-950 bp	1.0	86.2
ycf2-trnI-CAU_p4	GGAGCGAAACAATCAGCCTA	AGGTCCCATTCTTCTACCCC	50.0	55.0	58.0	58.1	1287-1296 bp	1.0	84.2
ycf2-trnI-CAU_p5	GGAGCGAAACAATCAGCCTA	GGTCCCATTCTTCTACCCCT	50.0	55.0	58.0	58.1	1286-1295 bp	1.0	84.2