Hotspot

psaC-ndhE

SSC · 131090-131443 · 354 bp

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Pi 0.0518

MarkerSeek score 79.20

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 354
variable_sites: 25
pi: 0.0518
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	CGGACACATTGAGTACACCC	TCGTAACAGAAAATCAACTCGT	55.0	36.4	58.3	56.1	415-462 bp	1.0	54.9
psaC-ndhE_p2	GGCTCGGACACATTGAGTAC	TCGTAACAGAAAATCAACTCGT	55.0	36.4	58.4	56.1	419-466 bp	1.0	54.3
psaC-ndhE_p3	GCTCGGACACATTGAGTACA	TCGTAACAGAAAATCAACTCGT	50.0	36.4	57.6	56.1	418-465 bp	1.0	54.2
psaC-ndhE_p4	AGGCTCGGACACATTGAGTA	TCGTAACAGAAAATCAACTCGT	50.0	36.4	58.4	56.1	420-467 bp	1.0	53.6
psaC-ndhE_p5	TCGGACACATTGAGTACACC	TCGTAACAGAAAATCAACTCGT	50.0	36.4	57.3	56.1	416-463 bp	1.0	51.3