Hotspot

rpl32-trnL-UAG

SSC · 127089-127802 · 714 bp

Back to Plumbago

Pi 0.0626
MarkerSeek score 80.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
714
variable_sites
65
pi
0.0626
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CAAGATCTCTCTCGACCGGA CAGGAGCACTGCTTCCTAAG 55.0 55.0 58.3 58.0 828-867 bp 1.0 85.2
rpl32-trnL-UAG_p2 CAAGATCTCTCTCGACCGGA AGGAGCACTGCTTCCTAAGA 55.0 50.0 58.3 58.1 827-866 bp 1.0 85.0
rpl32-trnL-UAG_p3 GGCTTTTCGGCAGCATTAAA CAGGAGCACTGCTTCCTAAG 45.0 55.0 57.6 58.0 862-901 bp 1.0 84.8
rpl32-trnL-UAG_p4 CAAGATCTCTCTCGACCGGA TGACCCGAATAAATCCAGCG 55.0 50.0 58.3 58.1 1189-1228 bp 1.0 83.2
rpl32-trnL-UAG_p5 GGCTTTTCGGCAGCATTAAA TGACCCGAATAAATCCAGCG 45.0 50.0 57.6 58.1 1223-1262 bp 1.0 82.8