Hotspot

ndhF-rpl32

SSC · 125855-126923 · 1069 bp

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Pi 0.0578

MarkerSeek score 81.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1069
variable_sites: 87
pi: 0.0578
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGGAAAAGACCACATACGCC	AATCCCCATCCCAGAACTGA	50.0	50.0	57.9	58.0	1595-1627 bp	1.0	88.9
ndhF-rpl32_p2	TTTGCTGCAATAGGTCGTGT	AATCCCCATCCCAGAACTGA	45.0	50.0	58.1	58.0	1984-2016 bp	0.666667	72.2
ndhF-rpl32_p3	ATCCCCCACACGATTAGTCA	AATCCCCATCCCAGAACTGA	50.0	50.0	58.1	58.0	2038-2056 bp	0.666667	69.5
ndhF-rpl32_p4	ATGAAGGGGAAATTGGGCAG	AATCCCCATCCCAGAACTGA	50.0	50.0	58.1	58.0	2221-2239 bp	0.666667	69.2
ndhF-rpl32_p5	GAAGGGGAAATTGGGCAGAT	AATCCCCATCCCAGAACTGA	50.0	50.0	57.8	58.0	2219-2237 bp	0.666667	68.9