Hotspot

rrn16-trnI-GAU

IRb · 110098-110389 · 292 bp

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Pi 0.0616

MarkerSeek score 81.30

Primer available yes

Label rrn16-trnI-GAU

Feature Metadata

label_name: rrn16-trnI-GAU
feature_type: igs
region: IRb
length_bp: 292
variable_sites: 27
pi: 0.0616
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-trnI-GAU_p1	CAAGGTAGCCGTACTGGAAG	CGGATGGGCACATTGAACTA	55.0	50.0	57.7	58.0	434-435 bp	1.0	84.5
rrn16-trnI-GAU_p2	AGTGACTGGAGTGAAGTCGT	CGGATGGGCACATTGAACTA	50.0	50.0	58.3	58.0	456-457 bp	1.0	84.1
rrn16-trnI-GAU_p3	ACAAGGTAGCCGTACTGGAA	CGGATGGGCACATTGAACTA	50.0	50.0	58.4	58.0	435-436 bp	1.0	84.0
rrn16-trnI-GAU_p4	AAGTCGTAACAAGGTAGCCG	CGGATGGGCACATTGAACTA	50.0	50.0	57.6	58.0	443-444 bp	1.0	83.9
rrn16-trnI-GAU_p5	AACAAGGTAGCCGTACTGGA	CGGATGGGCACATTGAACTA	50.0	50.0	58.4	58.0	436-437 bp	1.0	83.9