Hotspot

trnI-CAU-ycf2

IRb · 94099-94508 · 410 bp

Back to Plumbago

Pi 0.0618
MarkerSeek score 79.70
Primer available yes
Label trnI-CAU-ycf2

Feature Metadata

label_name
trnI-CAU-ycf2
feature_type
igs
region
IRb
length_bp
410
variable_sites
36
pi
0.0618
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-CAU-ycf2_p1 GTTGGGCGCTTTAACCATTC TTGAAGTGGGTCCATGAAGC 50.0 50.0 58.0 58.1 573-582 bp 1.0 90.3
trnI-CAU-ycf2_p2 GTTGGGCGCTTTAACCATTC GGAGCGAAACAATCAGCCTA 50.0 50.0 58.0 58.0 896-905 bp 1.0 86.9
trnI-CAU-ycf2_p3 GTTGGGCGCTTTAACCATTC CCAGGAAACAACTCTCCGAG 50.0 55.0 58.0 57.9 941-950 bp 1.0 86.1
trnI-CAU-ycf2_p4 AGGTCCCATTCTTCTACCCC GGAGCGAAACAATCAGCCTA 55.0 50.0 58.1 58.0 1287-1296 bp 1.0 84.2
trnI-CAU-ycf2_p5 GGTCCCATTCTTCTACCCCT GGAGCGAAACAATCAGCCTA 55.0 50.0 58.1 58.0 1286-1295 bp 1.0 84.2