Hotspot

ycf2

IRb · 86387-93235 · 6849 bp

Back to Pluchea

Pi 0.000146

MarkerSeek score 52.60

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRb
length_bp: 6849
variable_sites: 1
pi: 0.000146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	AGCAGAAGAGCCGTTTTCAA	AACTGTGGATCATTCGGACC	45.0	50.0	58.0	57.6	238 bp	1.0	76.8
ycf2_p2	AGCAGAAGAGCCGTTTTCAA	ACTGTGGATCATTCGGACCT	45.0	50.0	58.0	58.4	237 bp	1.0	76.7
ycf2_p3	ATGAGCAGAAGAGCCGTTTT	AACTGTGGATCATTCGGACC	45.0	50.0	57.8	57.6	241 bp	1.0	75.6
ycf2_p4	ATGAGCAGAAGAGCCGTTTT	ACTGTGGATCATTCGGACCT	45.0	50.0	57.8	58.4	240 bp	1.0	75.4
ycf2_p5	GCAGAAGAGCCGTTTTCAAG	AACTGTGGATCATTCGGACC	50.0	50.0	57.7	57.6	237 bp	1.0	74.5