Hotspot

rpoC2

LSC · 19045-23190 · 4146 bp

Back to Pluchea

Pi 0.000482

MarkerSeek score 54.10

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4146
variable_sites: 2
pi: 0.000482
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TGGAGGTACTGATGGCAGAA	CAGCGTCTTGAACTAGCCAT	50.0	50.0	58.0	58.0	237 bp	1.0	79.8
rpoC2_p2	GAGGTACTGATGGCAGAACG	CAGCGTCTTGAACTAGCCAT	55.0	50.0	58.1	58.0	235 bp	1.0	79.6
rpoC2_p3	TGGAGGTACTGATGGCAGAA	TTCAGCGTCTTGAACTAGCC	50.0	50.0	58.0	57.9	239 bp	1.0	79.3
rpoC2_p4	GAGGTACTGATGGCAGAACG	TTCAGCGTCTTGAACTAGCC	55.0	50.0	58.1	57.9	237 bp	1.0	79.1
rpoC2_p5	ATGGAGGTACTGATGGCAGA	CAGCGTCTTGAACTAGCCAT	50.0	50.0	57.8	58.0	238 bp	1.0	78.8