Hotspot

rpoC1

LSC · 16120-18943 · 2824 bp

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Pi 0

MarkerSeek score 56.00

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2824
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	TCTGAGAAGACTTTCCAGCT	GTACCTCCATATTGCGCTGA	45.0	50.0	55.8	57.5	2978-2979 bp	1.0	58.2
rpoC1_p2	TCTGAGAAGACTTTCCAGCT	TCAGTACCTCCATATTGCGC	45.0	50.0	55.8	57.5	2981-2982 bp	1.0	58.2
rpoC1_p3	TCTGAGAAGACTTTCCAGCT	AGTACCTCCATATTGCGCTG	45.0	50.0	55.8	57.5	2979-2980 bp	1.0	58.1
rpoC1_p4	TCTGAGAAGACTTTCCAGCT	CAGTACCTCCATATTGCGCT	45.0	50.0	55.8	57.5	2980-2981 bp	1.0	58.1
rpoC1_p5	TCTGAGAAGACTTTCCAGCTT	GTACCTCCATATTGCGCTGA	42.9	50.0	56.6	57.5	2978-2979 bp	1.0	56.5