Hotspot

ccsA

SSC · 122117-123085 · 969 bp

Back to Pluchea

Pi 0.00103

MarkerSeek score 59.30

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 1
pi: 0.00103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GCCGGTTGAGATAAGGTGTT	GCGGCATGCCATCTTCTAAA	50.0	50.0	57.9	59.0	1157 bp	1.0	71.4
ccsA_p2	TGATTCACATGGTTCTCGCC	GCGGCATGCCATCTTCTAAA	50.0	50.0	58.3	59.0	1174 bp	1.0	70.2
ccsA_p3	GTTCTCGCCGGTTGAGATAA	GCGGCATGCCATCTTCTAAA	50.0	50.0	57.7	59.0	1163 bp	1.0	70.0
ccsA_p4	ACCCCGTGAATCACTACACT	GCGGCATGCCATCTTCTAAA	50.0	50.0	58.4	59.0	1197 bp	1.0	69.4
ccsA_p5	GCCGGTTGAGATAAGGTGTT	GCGGCATGCCATCTTCTAA	50.0	52.6	57.9	58.3	1157 bp	1.0	68.8