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psbH-petB

LSC · 74713-75603 · 891 bp

Back to Pluchea

Pi 0.00113

MarkerSeek score 59.90

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: LSC
length_bp: 891
variable_sites: 1
pi: 0.00113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	GTCGCAATGGCTCTATTTGC	ATATCATCCGCAATCGCCTG	50.0	50.0	57.9	58.3	1071-1076 bp	1.0	77.4
psbH-petB_p2	GTCGCAATGGCTCTATTTGC	TCTCGAGACGCTCTTCAAAC	50.0	50.0	57.9	57.7	1049-1054 bp	1.0	76.9
psbH-petB_p3	GTCGCAATGGCTCTATTTGC	CCCCCTCCGAGAACTGTATA	50.0	55.0	57.9	57.6	997-1002 bp	1.0	76.6
psbH-petB_p4	CTTTGATGGGTGTCGCAATG	ATATCATCCGCAATCGCCTG	50.0	50.0	57.7	58.3	1082-1087 bp	1.0	76.1
psbH-petB_p5	CTTTGATGGGTGTCGCAATG	TCTCGAGACGCTCTTCAAAC	50.0	50.0	57.7	57.7	1060-1065 bp	1.0	75.6