Hotspot

ycf3-trnS-GCU

LSC · 44066-44977 · 912 bp

Back to Pluchea

Pi 0.0011

MarkerSeek score 59.40

Primer available yes

Label ycf3-trnS-GCU

Feature Metadata

label_name: ycf3-trnS-GCU
feature_type: igs
region: LSC
length_bp: 912
variable_sites: 1
pi: 0.0011
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GCU_p1	TTCTCCTGAAGTTGTCGGAA	AAGCAAACGCCTACATAGCA	45.0	45.0	56.4	57.9	1067 bp	1.0	66.5
ycf3-trnS-GCU_p2	TTTCTCCTGAAGTTGTCGGA	AAGCAAACGCCTACATAGCA	45.0	45.0	56.4	57.9	1068 bp	1.0	66.5
ycf3-trnS-GCU_p3	TTCTCCTGAAGTTGTCGGAA	ATGCTACGCTTTCAACCACT	45.0	45.0	56.4	57.8	1041 bp	1.0	65.9
ycf3-trnS-GCU_p4	TTTCTCCTGAAGTTGTCGGA	ATGCTACGCTTTCAACCACT	45.0	45.0	56.4	57.8	1042 bp	1.0	65.9
ycf3-trnS-GCU_p5	TTCTCCTGAAGTTGTCGGAA	GGATTCGAACCCTCGGTAAG	45.0	55.0	56.4	57.8	1084 bp	1.0	65.6