Hotspot

psbA-matK

LSC · 1553-2088 · 536 bp

Back to Pluchea

Pi 0

MarkerSeek score 57.60

Primer available yes

Label psbA-matK

Feature Metadata

label_name: psbA-matK
feature_type: igs
region: LSC
length_bp: 536
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-matK_p1	TAAAGACGGTTTTCGGTGCT	CGCGAAGGTTATCTAGAAGGC	45.0	52.4	57.8	58.6	695-696 bp	1.0	65.3
psbA-matK_p2	TAAAGACGGTTTTCGGTGCT	GCGAAGGTTATCTAGAAGGCG	45.0	52.4	57.8	58.6	694-695 bp	1.0	65.3
psbA-matK_p3	GTAAAGACGGTTTTCGGTGC	CGCGAAGGTTATCTAGAAGGC	50.0	52.4	57.7	58.6	696-697 bp	1.0	64.8
psbA-matK_p4	GTAAAGACGGTTTTCGGTGC	GCGAAGGTTATCTAGAAGGCG	50.0	52.4	57.7	58.6	695-696 bp	1.0	64.8
psbA-matK_p5	TTTTCGGTGCTGGTTATCCA	GCGAAGGTTATCTAGAAGGCG	45.0	52.4	57.4	58.6	685-686 bp	1.0	62.7