Hotspot

DXZ06_pgt001-psbA

LSC · 75-490 · 416 bp

Back to Pluchea

Pi 0.0144
MarkerSeek score 63.20
Primer available yes
Label DXZ06_pgt001-psbA

Feature Metadata

label_name
DXZ06_pgt001-psbA
feature_type
igs
region
LSC
length_bp
416
variable_sites
6
pi
0.0144
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
DXZ06_pgt001-psbA_p1 GATCCACTTGGCTACATCCG CCGTGCTAACCTTGGTATGG 55.0 55.0 58.1 58.3 535 bp 1.0 77.2
DXZ06_pgt001-psbA_p2 CAATCCACTGCCTTGATCCA CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 549 bp 1.0 77.1
DXZ06_pgt001-psbA_p3 AATCCACTGCCTTGATCCAC CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 548 bp 1.0 77.1
DXZ06_pgt001-psbA_p4 ACAATCCACTGCCTTGATCC CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 550 bp 1.0 77.1
DXZ06_pgt001-psbA_p5 GATCCACTTGGCTACATCCG ACCGTGCTAACCTTGGTATG 55.0 50.0 58.1 57.6 536 bp 1.0 76.7