Hotspot

ycf1

SSC · 113281-118896 · 5616 bp

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Pi 0.0127
MarkerSeek score 45.50
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5616
variable_sites
71
pi
0.0127
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TCCAGAAACCGAACAAGGAA GATGATCCTCTGCCACGTTT 45.0 50.0 57.0 58.0 212 bp 1.0 73.9
ycf1_p2 TTCCAGAAACCGAACAAGGA GATGATCCTCTGCCACGTTT 45.0 50.0 57.0 58.0 213 bp 1.0 73.9
ycf1_p3 TCCAGAAACCGAACAAGGAA GATCCTCTGCCACGTTTTCT 45.0 50.0 57.0 57.9 209 bp 1.0 73.4
ycf1_p4 TTCCAGAAACCGAACAAGGA GATCCTCTGCCACGTTTTCT 45.0 50.0 57.0 57.9 210 bp 1.0 73.4
ycf1_p5 TCCAGAAACCGAACAAGGAA TCCTCTGCCACGTTTTCTTC 45.0 50.0 57.0 58.1 207 bp 1.0 73.2