Hotspot

ccsA-trnL-UAG

SSC · 128541-128653 · 113 bp

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Pi 0.0354
MarkerSeek score 69.30
Primer available yes
Label ccsA-trnL-UAG

Feature Metadata

label_name
ccsA-trnL-UAG
feature_type
igs
region
SSC
length_bp
113
variable_sites
4
pi
0.0354
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-trnL-UAG_p1 ACAATTGAAACGATCGAAAAGGA GTGAAATCGGTAGACACGCT 34.8 50.0 57.3 58.0 246 bp 1.0 51.8
ccsA-trnL-UAG_p2 ACAATTGAAACGATCGAAAAGGA TGAAATCGGTAGACACGCTG 34.8 50.0 57.3 58.0 245 bp 1.0 51.8
ccsA-trnL-UAG_p3 ACAATTGAAACGATCGAAAAGGA TAGACACGCTGCTCTTAGGA 34.8 50.0 57.3 57.9 236 bp 1.0 50.9
ccsA-trnL-UAG_p4 ACAATTGAAACGATCGAAAAGGA TTTCGAGTATGCCGCTATGG 34.8 50.0 57.3 57.9 265 bp 1.0 50.6
ccsA-trnL-UAG_p5 ACAATTGAAACGATCGAAAAGGA GTAGACACGCTGCTCTTAGG 34.8 55.0 57.3 57.8 237 bp 1.0 50.3