Hotspot

ndhI-ndhG

SSC · 123624-124041 · 418 bp

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Pi 0.0144
MarkerSeek score 63.20
Primer available yes
Label ndhI-ndhG

Feature Metadata

label_name
ndhI-ndhG
feature_type
igs
region
SSC
length_bp
418
variable_sites
6
pi
0.0144
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhI-ndhG_p1 ATCAAATCGCTTTGGGTCGT ATTGGTCCAGGCAAATCCAT 45.0 45.0 58.2 57.4 512-518 bp 1.0 75.1
ndhI-ndhG_p2 TCAAATCGCTTTGGGTCGTT ATTGGTCCAGGCAAATCCAT 45.0 45.0 58.4 57.4 511-517 bp 1.0 73.3
ndhI-ndhG_p3 CAAATCGCTTTGGGTCGTTT ATTGGTCCAGGCAAATCCAT 45.0 45.0 57.6 57.4 510-516 bp 1.0 73.2
ndhI-ndhG_p4 ATCAAATCGCTTTGGGTCGT TCATGTATTGGTCCAGGCAA 45.0 45.0 58.2 56.8 518-524 bp 1.0 70.0
ndhI-ndhG_p5 ATCAAATCGCTTTGGGTCGT TATTGGTCCAGGCAAATCCA 45.0 45.0 58.2 56.8 513-519 bp 1.0 69.8